Counting colonies of clonogenic assays by using densitometric software
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Counting colonies of clonogenic assays by using densitometric software
Clonogenic assays are a useful tool to test whether a given cancer therapy can reduce the clonogenic survival of tumour cells. A colony is defined as a cluster of at least 50 cells which can often only be determined microscopically. The process of counting colonies is very extensive work and so we developed software that is able to count the colonies automatically from scanned flasks. This soft...
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MALLIGO, JOHN E. (Fort Detrick, Frederick, Md.). Evaluation of an automatic electronic device for counting bacterial colonies. Appl. Microbiol. 13:931-934. 1965.-An automatic colony counter was tested extensively with colonies of two bacterial species, Serratia marcescens and Bacillus subtilis var. niger, grown on agar media. A stable relationship was established between machine counts and coun...
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Bacterial colony enumeration is an essential tool for many widely used biomedical assays. This chapter introduces a cost-effective and fully automatic bacterial colony counter which accepts digital images as its input. The proposed counter can handle variously shaped dishes/plates, recognize chromatic and achromatic images, and process both color and clear medium. In particular, the counter can...
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This paper examines the efficacy of liver cancer treatment using HA22T cancer cells specific to the Taiwanese population. The Clonogenic Assay is the current standard method for detecting liver cancer. This paper uses image processing technology and a fuzzy inference system to identify in-vitro colonies of cancer cells. A scanner was first used to capture an image of the culture dish. This imag...
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Investigating the effect of low-dose radiation exposure on cells using assays of colony-forming ability requires large cell samples to maintain statistical accuracy. Manually counting the resulting colonies is a laborious task in which consistent objectivity is hard to achieve. This is true especially with some mammalian cell lines which form poorly defined or 'fuzzy' colonies, typified by glio...
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ژورنال
عنوان ژورنال: Radiation Oncology
سال: 2007
ISSN: 1748-717X
DOI: 10.1186/1748-717x-2-4